1H MRSI Revealed Influence of Genetic Background on Neuronal Behavior in Murine Model of HIV-1 Encephalitis
Gendelman H, Gorantla S, Mellon M, Bradley J, Kibuule D, Dou H, Poluektova L, Boska M, Nelson J
University of Nebraska Medical Center, University of Nebraska Medical Center
Murine models of human immunodeficient virus type-1 encephalitis (HIVE), C.B.-17/SCID and Rag-2-/-&[gamma]c-/-, were compared for their ability to mimic human pathology. Mice were injected in the subcortex with infected human monocyte derived macrophages (MDM) and data were collected 7-days post-infection. Compared to Rag-2-/-&[gamma]c-/-, C.B.-17/SCID mice showed increased microglia activation, astrocytosis, neuronal damage, TNF-&[alpha] and IL-1&[beta], enhanced chemoattraction of peripheral MDM and reduced MDM numbers in brain tissue. C.B.-17/SCID also had reduced concentrations of NAA/Cre globally. The increased and widely distributed inflammatory responses with increased neuronal damage in the C.B.-17/SCID model better mimics HIVE pathology than the Rag-2-/-&[gamma]c-/- murine model.