Labeling and Imaging Stem/Progenitor Cells with Multiple Unique Nanoparticulate Fluorine Markers: The Potential for Multispectral Stem Cell Detection with 19F MRI
Brant J, Neubauer A, Lanza G, Caruthers S, Wickline S, Crowder K, Chen J
We and others have shown previously that 19F MRI of liquid perfluorocarbon (PFC) nanoparticles may present a unique and sensitive cell label (fluorine) that cannot be obscured by traditional tissue background proton signal. In the present study, we aimed to explore the possibility of creating multiple unique and differentiable PFC labels for stem cells. Our results showed that perfluoro-15-crown-5 ether and perfluorooctylbromide nanoparticles could serve as simultaneous intracellular markers with the use of 19F MRS/MRI at 11.7T.