MRI of Neural Progenitor Migration in the Developing Mouse Brain
Wadghiri Y, Deans A, Turnbull D
New York University School of Medicine
Micron-sized particles of iron oxide (MPIO) have recently been shown to label stem cells in culture and in vivo. We used MPIO to label neural progenitor cells in situ in the medial and lateral ganglionic eminences of the embryonic mouse forebrain. Five days after labeling, T2*-MRI at 7T and histology of ex vivo samples demonstrated that neuronal progenitors had taken up particles and migrated to the expected locations in the cortex and striatum. These results show promise for this approach in future in vivo cell migration studies in the embryonic mouse brain.