Fluorine Diffusion Measurements Confirm Intracellular Labeling of Perflurocarbon Nanoparticles in Therapeutic Stem/Progenitor Cells as Tracking Agents
Chen J, Crowder K, Brant J, Lanza G, Wickline S
The ability to track transplanted therapeutic cells in-vivo is critical for elucidating mechanisms of cell migration and differentiation. 19F MRI of liquid perfluorocarbon nanoparticles (PFC NP: 200 nm) may offer a unique and sensitive cell marker (fluorine) that cannot be obscured by traditional tissue background proton signal. We proposed that 19F diffusion weighted MRS/MRI could provide a non-invasive method to confirm intracellular labeling of stem/progenitor cells by specifically detecting intracellular PFC NP as its diffusivity decreases dramatically upon successful cell labeling.