T2-labelling of phosphate by 17O a method with potential for in vivo studies of phosphorus metabolism
University of Florida
As 31P has 100% natural abundance and is the only MR-visible stable isotope of phosphorus, tracer studies analogous to those employed with 13C are not appicable to MR studies of the metabolism of phosphorus-containing compounds. Previous studies have labelled phosphate with 17O or 18O, detecting the presence of these isotopes by mass spectrometry or by the isotope effect of 18O on the 31P chemical shift. However, neither of these methods is applicable to in vivo studies. We present a method to detect 17O-labelled phosphate via the scalar coupling interaction between 31P and 17O, which is applicable to in vivo studies.