Separating Intra- and Extracellular Water Signals in Rat Brain Using Gd-DPTA-Enhanced Diffusion-Weighted MRI
Thelwall P, Sotak C, Shepherd T, Blackband S
University of Florida
This study investigated trasmembrane water exchange in viable rat cortical brain slices with previously described methods that employ Gd-DPTA to alter the T1 properties of extracellular water and thus resolve the intra- and extracellular water signals using diffusion-weighted inversion recovery pulse sequences. Data obtained suggest that Gd-DTPA concentrations exceeding 8 mM are needed for transmembrane water exchange to be in an intermediate or slow regime relative to the T1 relaxation rate.