Diagnosis of Escherichia coli induced urinary tract infection by NMR
Jain A, Nagana Gowda G, Gupta A, Roy R, Bhandari M, Khetrapal C, Dwivedi M, Mehdi A
Department of Microbiology
1H NMR spectroscopic method is suggested and applied for the diagnosis of Escherichia coli (E. coli) in the urinary tract infection (UTI). Specific property of E. coli of metabolizing lactose to lactate in urine is exploited. Other common bacteria, such as Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterobacter aerogenes, Acinetobacter baumanii, Proteus mirabilis, Citrobacter frundii do not produce lactate. The method was applied to E. coli (n=96) infected urine samples and the results showed very high sensitivity with reference to conventional method. Diagnosis of E. coli bacteria causing UTI infection in a single step both qualitatively and quantitatively may have useful implications.