Continuous Saturation Correction in the Presence of Changing Metabolite Concentrations
Cole M, Carr C, Stuckey D, Clarke K, Tyler D
University of Oxford
Accurate quantitation of 31P metabolites requires rapid acquisition of many averages. Correction for saturation is then needed. However, correction can be prone to errors if the effective T1 changes during the course of an experiment, as in an ischaemia-reperfusion experiment. In this work we have implemented a continuous saturation correction scheme based on the dual-angle method. We have demonstrated the feasibility of the technique throughout an ischaemia-reperfusion experiment, even in the presence of an ~75% increase in the effective T1 of one of the metabolites. The experiments were performed in the same time as a normal spectroscopic experiment.