Lijing Xin1, Bernard Lanz1, Hanne Frenkel1, Rolf Gruetter1,2
1Laboratory of functional and metabolic imaging, Ecole Polytechnique Federale de Lausanne (EPFL), Lausanne, Switzerland; 2Department of Radiology, University of Lausanne , Lausanne, Switzerland
Indirect detection of 13C label through proton offers higher sensitivity but suffers from lower spectral resolution, such as the GluC3 and GlnC3 region. A novel 1H-[13C] sequence combining SPECIAL localization and BISEP pulse was proposed and applied in vivo during infusion of the glia-specific substrate - [2-13C] acetate at 14.1T. The results showed the separate measurement of GluC4, GlnC4, GlnC3 and GluC3 time courses with high temporal resolution at 14.1T, which greatly enhanced the ability to study neuron-glial metabolism using 1H-[13C] NMR spectroscopy.