Sachchidanand Srivastava1, Rishi Awasthi2, Namdeo Sri Ram Gajbhiye1, Vikas Agarwal3, Amit Singh3, Rakesh K. Gupta
1Department of Chemistry, Indian Institute of Technology, Kanpur, Uttar Pradesh, India; 2Department of Radiodiagnosis, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India; 3Department of Immunology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India
The synthesis of monodispersive citrate coated Fe3O4 magnetic nanoparticle was done by high temperature co-precipitation method using diethyl glycol as solvent. The nanoparticle was characterized by Powder X-ray Diffraction (XRD), transmission electron microscopy (TEM), Fourier Transform Infra Red (FTIR) and Vibrational Sample Magnetometer (VSM). Cellular uptake of magnetic nanoparticles was found to be 90-95% in RAW cell line and 20-30% in jurket cell line. Jurket cell line being non-phagocytic, showed less uptake of magnetic nanoparticles, but the uptake may indicate the nonspecific engulfment of these nanoparticles. On T2*GRE sequence the labeled cells showed strong T2* effect. We conclude that the electrostatic stabilization allows the production of much smaller nano particles than conventional USPIO and on proper optimization these nanoparticles may be used as MR contrast agent.