Wen-Tung Wang1, Sang-Pil Lee2, Elias Michaelis3, In-Young Choi1,4
1Hoglund Brain Imaging Center, University of Kansas Medical Center, Kansas City, KS, USA; 21Hoglund Brain Imaging Center, University of Kansas Medical Center, Kansas City, KS, USA; 3Department of Pharmacology and Toxicology, University of Kansas, Lawrence, KS, USA; 4Department of Neurology, Molecular & Integrative Physiology, Hoglund Brain Imaging Center, Kasnas City, KS, USA
Localized in vivo 1H magnetic resonance spectroscopy (MRS) was used to study hippocampal and striatal metabolite levels in the transgenic mice, who have mutation in glutamate dehydrogenase 1 (Glud1) and thus are hypersensitive to ethanol, as well as age-matched wild type mice. MRS data were acquired from hippocampus and striatum in the brain of both groups before and after ethanol diet. Our results show that there were small changes between the neurochemical profiles of the wt and the Glud1 tg mice, especially for metabolites Glu, Gln, and Asp linked to Glu metabolism, suggesting a modest effect of Glud over-expressing on amino acid homeostasis. With 2-week ethanol diet, the Gln level had significant changes for both brain regions in both wt and Glud tg mice. It remains to be determined whether Glud over-expressing will exacerbate the chronic effect of alcohol on the brain.