Niels Braakman1, Firat Kara1, Mark A. van Buchem2, Reinhard Schliebs3, Huub J.M. de Groot1, A Alia1
1Leiden Institute of Chemistry, Leiden University, Leiden, P.O. Box 9502, Netherlands; 2Department of Radiology, Leiden University Medical Center, Leiden, Netherlands; 3Paul Flechsig Institute for Brain Research, University of Leipzig, Leipzig, Germany
In this study we employed in vivo localized two dimensional (2D) MRS to map the neurochemical changes in transgenic mouse model of AD as compared to control mouse. The combination of the optimized 2D L-COSY sequence, high field strength (9.4T) and strong gradient system allowed clear detection of cross-peaks of several brain metabolites allowing their direct unambiguous chemical shift assignments in vivo. Significant changes in various neurometabolites are evident in 2D spectra obtained from the brain of transgenic mice as compared to control mice. The neurochemical changes observed in AD brain and their relation with AD pathology will be discussed.