Brige Paul Chugh1, Jason P. Lerch1, Lisa X. Yu1, R Mark Henkelman1, John G. Sled1
1Toronto Centre for Phenogenomics, Mouse Imaging Centre, The Hospital for Sick Children, Toronto, Ontario, Canada
We developed an efficient imaging protocol to study the relationship between cerebral blood volume (CBV) and cerebral blood flow (CBF) over regions of the entire mouse brain, which may in future be applied to phenotype models of neurovascular disease. We performed a study that compared normative values of CBV and CBF over 13 mouse brain regions and over the cerebral cortex. The CBF was imaged using 3D arterial spin labeling and these images were co-registered to CBV images determined by a micro-CT protocol. This method could be useful for characterizing regional differences in vascularity in mouse models of cerebrovascular disease.