Sven Gottschalk1, Dieter Leibfritz2, Claudia Zwingmann1,2, Marc Bilodeau1
1Dpartement de sciences biomdicales, Universit de Montral, Montral, Qubec, Canada; 2Department of Organic Chemistry, University of Bremen, Bremen, Germany
Application of stable isotope labeling in vitro is a potent technique to study metabolic pathways and fluxes. We applied [2-13C]pyruvate on primary mouse hepatocyte cultures to compare its usefulness for metabolic studies and flux analysis with other labeled substances. Our results show: [2-13C]pyruvate was metabolized by lactate dehydrogenase, alanine-/aspartate-aminotransferase, malic enzyme, PC, PDH and subsequent metabolic pathways through the TCA-cycle. Taken together with our previous findings, we conclude that [2-13C]pyruvate is a versatile physiological substrate to study hepatocellular pathways and the de novo synthesis of major metabolites in these cells under normal and pathological conditions.