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Abstract #4303

Proton NMR Spectroscopic Analysis of Pus for Differentiation Between Amoebic and Pyogenic Liver Abscesses

Santosh Kumar Bharti1, Virendra Jaiswal2, Ujjala Ghoshal2, Raja Roy1, S K. Mandal1, S S. Baijal3, U C. Ghoshal4, C L. Khetrapal1

1CBMR, Centre of Biomedical Magnetic Resonance, Lucknow, Uttar Pradesh, India; 2Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India; 3Radiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India; 4Gastroenterology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India


Hepatic abscesses with different types of infection such as bacterial and parasitic provide a challenging task for easier and early differentiation between such infections. PCR, Culture and 1H NMR analysis of pus were carried out on 118 patients with hepatic abscess. The 1H NMR spectroscopic analysis provided distinctive characterization of metabolites, viz., asparagine and aspartic acid in amoebic liver abscess, while pyogenic abscess showed the presence of acetate, succinate and formate. The NMR results reported herein have the advantage that the measurement can be rapidly performed in single step rather than two separate tests for bacterial and parasitic infections.