Praveen Kumar Gulaka1, Vikram D. Kodibagkar1,2, Jian-Xin Yu2, Ralph P. Mason, 12
1Biomedical Engineering, UT Arlington and UT Southwestern Medical Center at Dallas, Dallas, Tx, United States; 2Radiology, UT Southwestern Medical Center at Dallas, Dallas, Tx, United States
Extensive implementation of gene therapy as a therapeutic strategy for cancers has been hampered by difficulties in quantitatively assessing the success of gene transfection and longevity of gene expression. Therefore development of non-invasive reporter techniques based on appropriate molecules and imaging modalities may help to assay gene expression. We have evaluated a range of S-Gal analogs as novel 1H MR lacZ gene-reporter molecules in vitro and have identified C3-GD as an optimal agent for in vivo studies.