Ulrich Flgel1, Su Song2, Inga Kreidewei1, Zhaoping Ding1, Oliver Witzke2, Jrgen Schrader1
1Institut fr Herz- und Kreislaufphysiologie, Heinrich-Heine-Universitt, Dsseldorf, NRW, Germany; 2Klinik fr Nephrologie, Universittsklinikum Essen, Germany
This study was aimed at developing an approach for the early in vivo detection of organ rejection in a murine heterotopic abdominal heart transplantation model. As contrast agent emulsified perfluorocarbons (PFCs) were used, which are biochemically inert and are known to be phagocytized by monocytes/macrophages. 1H/19F MRI enabled us to detect the initial immune response not later than 3 days after surgery, when conventional parameters did not reveal any signs of rejection. The results show that intravenously applied PFCs accumulate in areas affected by rejection and can be sensitively detected by 1H/19F MRI at a field strength of 9.4 T.