Nicole Martina Adriana van den Broek1, Jolita Ciapaite1, Klaas Nicolay1, Jeanine J. Prompers1
1Biomedical NMR, Department of
Biomedical Engineering ,
The interpretation of basal ATP synthesis flux (VATP) measured by 31P saturation transfer (ST) is not straightforward. In this study, post-exercise PCr recovery and ST-based VATP were compared in a rat model of mitochondrial dysfunction. Treatment with complex 1 inhibitor DPI induced mitochondrial dysfunction, as evidenced by a decreased oxygen consumption rate in isolated mitochondria and a decreased in vivo post-exercise PCr recovery. Interestingly, no significant difference in VATP was observed between DPI-treated rats and controls. This shows that ST measurements in rest do not necessarily reflect intrinsic mitochondrial function, but more likely the ATP demand of the cell.