Duncan Jack Hodkinson1, Carmen de Groote2, Shane McKie3, John-Francis William Deakin3, Steve R. Williams1
1Imaging Science and Biomedical Engineering, University of Manchester, Manchester, United Kingdom; 2Neuroscience and Biomedical Systems, University of Glasgow, Glasgow, United Kingdom; 3Neuroscience and Psychiatry Unit, University of Manchester, Manchester, United Kingdom
Pharmacological-challenge MRI (phMRI) is an exciting new tool enabling researchers to examine underlying circuitry of the brain in response to neuroactive drugs. To avoid head movements pre-clinical phMRI studies are often conducted under general anaesthesia. However, interactions between the drug of interest and the anaesthetic may be a confounding factor. Here we assessed the effect of α-chloralose and isoflurane anesthesia on the phMRI response to ketamine challenge. The positive BOLD signal changes observed with α-chloralose showed areas of activation similar to neuroimaging studies in humans. A drug-anaesthetic interaction between isoflurane and ketamine compromised the phMRI response.