A Alia1,
S Kabli1, S He2, E S. Jagalska2, A Hurlstone3,
H P. Spaink2, H J. M de Groot1
1Leiden Institute of Chemistry, Leiden
University, Leiden, Netherlands; 2Institute of Biology, Leiden
University, Leiden, Netherlands; 3Faculty of Life Sciences,
University of Manchester, Manchester, United Kingdom
Zebrafish
cancer models are fast gaining ground in cancer research. Most tumors in
zebrafish develop late in life, when fish are no longer transparent, limiting
in vivo optical imaging methods. Thus, non-invasive imaging to track tumors
in adult zebrafish remains challenging. In this study tumors were visualized
in transgenic zebrafish using MRI at 9.4T.
Furthermore, live imaging of tumors at ultra-high field (17.6T)
revealed significant tumor heterogeneity. This study demonstrating the
application of μMRI to detect the locations, invasion status and
characteristics of internal melanomas in zebrafish and pave the way for
tracking tumor development and real-time assessment of therapeutic effects in
zebrafish tumor models.