Peter Andrew Hardy1, Luke H. Bradley1,
Zhiming Zhang1, Don Gash1, Dan Keeley2,
Brian Kramer3, Greg Schorn4
1Anatomy & Neurobiology, University
of Kentucky, Lexington, KY, United States; 2Advanced Technologies
and Regenerative Medicine, Rayntham, MA, United States; 3Advanced
Technologies and Regenerative Medicine, Somerville, NJ, United States; 4Advanced
Technologies and Regenerative Medicine, Rayntham, Massachussets, United
States
Convection
Enhanced Delivery is a powerful method of delivering drugs to the CNS.
MR-visible tracers co-infused with drug will be useful to assess drug
distribution. We tested four compounds (Magnevist and three Gd-labeled
polylysines) with molecular weights between 0.5k Da and 200 kDa as potential
tracers for CED. Compounds were tested in vitro to model CED parameters used
to plan delivery into four rhesus monkeys. In vitro results demonstrated MW
dependent CED distribution. In vivo results demonstrated distribution of the
Magnevist in the putamen but little distribution of the polylysine as a
result of binding and digestion of the polylysine.