David Peter Cormode1, Gitte Oskov Knudsen1, Amanda Delshad1, Nicole Parker2, Peter Jarzyna1, Torjus Skajaa3, Karen C. Briley-Saebo1, Ronald E. Gordon4, Zahi Adel Fayad1, Savio L C Woo2, Willem J M Mulder1
1Radiology, Mount Sinai School of Medicine, New York, NY, United States; 2Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY, United States; 3Clinical Institute and Dept. of Cardiology, Aarhus University, Skejby, Denmark; 4Pathology, Mount Sinai School of Medicine, New York, NY, United States
We have developed a fluorescent iron oxide nanoparticle platform with a gene transfection-enabling polymeric coating. This platform allows gene transfer to be studied via MRI, fluorescence and TEM imaging techniques. We have studied this platform in the setting of liver disease and the effect of varying the polymeric coating by increasing the PEG content from 0-25%. We found that the MRI and fluorescence contrast in the liver was unaffected by the particle coating, however, the cellular distribution was skewed from the Kupffer cells to the therapeutically relevant hepatocytes when the percentage of PEG was increased.