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Abstract #1876

The Changes of the Metabolite Profile as Human Mesenchymal Stem Cells Differentiate to Adipocytes Mesured by in Vitro 9.4T MR Spcetroscopy

Zhi-Feng Xu1, Chong-Yang Shen2, Lin-Ping Wu2, Ye-Yu Xiao, Yao-Wen Chen, Ren-Hua Wu

1medical imging, the 2nd Affiliated Hospital, the Medical College of Shantou University, shantou, guangdong, China; 2Multidisciplinary Research Center of Shantou University, shantou, guangdong, China


In this study, we attempt to study the alteration of metabolite of MSCs undergoing adipogenic differentiation to targeted fat cells in vitro, using 9.4T high-resolution 1H NMR spectroscopy.lastly, in our study,several major metabolites can be observed in the MR sepectroscopy that is before and after differentiation of MSCsincluding choline, creatine, glutamate and myo-inositol, acetate and some fatty acids,etc.Quantiļ¬cation of metabolite concentrations was performedthe levels of intracellular metabolites, such as choline, creatine, glumate and acetate all decreased, with the increased level of methionine , succinate and fatty acids after the MSCs differentiation 2 weeks.It indiactes that we can mintor differentiation of MSCs,according the changes of metabolites.