Rami Robert Hallac1, Vikram D. Kodibagkar1, Jian-Xin Yu1, Ralph P. Mason1
1Radiology, UT Southwestern
The detection of enzyme activity and gene expression in vivo is potentially important for the characterization of diseases and gene therapy. We demonstrate the use of a novel class of dual 1H/19F NMR lacZ gene reporter molecule to identify ]-gal expressing tumors in mice. The substrate shows a single 19F NMR signal and exposure to -galactosidase induces a large 19F NMR chemical shift response. The combination of Fe3+ and released aglycone product generates intense T2 contrast only in ]-gal expressing tumor. The dual modality approach allows both the detection of substrate and imaging of product.