Prachi Pandit1,2, Yi Qi2, Kevin
F. King3, G A. Johnson1,2
1Biomedical Engineering, Duke
University, Durham, NC, United States; 2Center for In Vivo
Microscopy, Duke University, Durham, NC, United States; 3GE
Healthcare, Waukesha, WI, United States
The
requirements for preclinical cancer imaging are high spatial resolution, good
soft tissue differentiation, excellent motion immunity, and fast and
non-invasive imaging to enable high-throughput, longitudinal studies. Here we
describe a PROPELLER-based technique, which with its unique data acquisition
and reconstruction overcomes the adverse effects of physiological motion,
allows for rapid setup and acquisition and provides excellent tissue
contrast. Hardware optimization as well as sequence modification enable us to
obtain heavily T2-weighted images at high-fields in tumor-bearing mice with
in-plane resolution of 117μm and slice thickness of 1mm. Multi-slice
datasets covering the entire thorax and abdomen are acquired in ~40 minutes.