Karlos X. Moreno1, Crystal E. Harrison1,
Matthew E. Merritt1, Zoltan Kovacs1, Zengdun Shi2,
Don C. Rockey2, A Dean Sherry1, Craig R. Malloy1,2
1Advanced Imaging Research Center, Univ
of TX Southwestern Med Ctr, Dallas, TX, United States; 2Internal
Medicine, Univ of TX Southwestern Med Ctr, Dallas, TX, United States
Pentose
phosphate pathway flux was studied using hyperpolarized δ-[1-13C]gluconolactone
injected into an isolated perfused mouse liver. Control livers produced a significant
amount of H13CO3-, a product indicative of
pentose phosphate pathway flux and [1-13C]gluconate. Hydrogen peroxide damaged livers also
produced H13CO3- and [1-13C]gluconate,
though the bicarbonate was at lower amounts than the control. CCl4 treated livers did not
produce any observable H13CO3-, but [1-13C]gluconate
was produced. These studies show that
the lactone is incorporated within the hepatocyte, phosphorylated and
metabolized through the pentose phosphate pathway.