NMR Investigations of
Neuronal and Astroglial Metabolism in Nicotine Addiction
Anant Bahadur Patel1, Mohammad Shameem1
1NMR Microimaging and Spectroscopy,
Centre for Cellular and Molecular Biology, Hyderabad,
Andhra Pradesh, India
this study we have used a novel approach of co-infusion of [U-13C6]glucose
and [2-13C]acetate to investigate neuronal and astroglial
metabolism in nicotine addiction. C57BL6 mice were injected (s.c.) with nicotine
three times a day for a month. Measurements were carried out 2 days after the
last treatment by infusing [2-13C]acetate and [U-13C6]glucose
for 20 min. 13C Labeling of amino acids were measured with 1H-[13C]-
and 13C-[1H]-NMR spectroscopy at 14T NMR spectrometer.
Nicotine treatment did not alter the cerebral metabolites levels in the
different brain regions. However, glutamatergic and GABAergic rate were
increased in cortex with chronic nicotine treatment.