Daniel James Stuckey1, Hikaru Ishii2, Aldo R. Boccaccini2, Carolyn A. Carr1, Judith A. Roether2, Qi Zhi Chen2, Hedeer Jawad2, Damian J. Tyler1, Nadire N. Ali2, Kieran Clarke1, Sian E. Harding2
1Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, Oxon, United Kingdom; 2National Heart and Lung Institute, Imperial College, London, United Kingdom
MRI was used to test three different scaffold materials designed for myocardial tissue engineering. Scaffold location, degradation and effect on cardiac function were measured in vivo at 1 and 6 weeks after grafting of scaffold onto infarcted rat hearts. The rigid TiO2-PED scaffold induced microvascular occlusion and necrosis adjacent to the scaffold, resulting in reduced cardiac function by six weeks. The PGS scaffold was not detrimental to function, but MRI showed that the material degraded between 1 and 6 weeks in vivo. This study demonstrates the feasibility and importance of using MRI to optimise myocardial tissue engineering strategies.