Ming-Hung Chen1, Gin-Chung Liu2,3, Twei-Shiun Jaw2,4, Yu-Ting Kuo2,3, Chiao-Yun Chen2,3, Yun-Ming Wang1
1Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan; 2Department of Medical Imaging, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; 3Department of Radiology,Faculty of Medicine,College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; 4Department of Radiology,Faculty of Medicine,College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
In this study, the various core sizes of manganese ferrite nanoparticles (MnFe2O4) conjugated with D4 peptide (MnFe2O4-PEG-D4) were synthesized. The high relaxivity MnFe2O4 nanoparticles were obtained by thermal decomposition of Iron acetylacetonate and manganese acetylacetonate in hydrophobic solution at high boiling process. The surface of MnFe2O4 nanoparticles were coated with polyethylene glycol (PEG) and EGFR peptide ligand (D4: Leu-Ala-Arg-Leu-Leu-Thr) to improve their dispersion and ability to target EGFR. The negative signal enhancement of EGFR expressing cancer cells (SKBR-3 and PC-3) were significantly higher than that of low EGFR expressing cells (HEK-293).