Helmar Waiczies1,2, Bettina Erdmann3, Bernd Ittermann1,2, Frank Seifert1,2, Thoralf Niendorf, 2,4, Sonia Waiczies, 2,5
1Physikalisch Technische Bundesanstalt, 10587 Berlin, Germany; 2Berlin Ultrahigh Field Facility, Max-Delbrueck Center for Molecular Medicine, 13125 Berlin, Germany; 3Electron Microscopy, Max-Delbrueck Center for Molecular Medicine, 13125 Berlin, Germany; 4Experimental and Clinical Research Center (ECRC), Charit Campus Buch, Humboldt-University, 13125 Berlin, Germany; 5Department of Hematology and Oncology, Charit Campus Buch, Humboldt-University, 13125 Berlin, Germany
19F cellular magnetic resonance imaging (MRI) provides signal selectivity during cell tracking and a possibility to overlay 19F-labeled cells with anatomic 1H scans. This work investigates the uptake of nanoparticles containing perfluoro-15-crown-5 ether in dendritic cells and their impact on cell function. 19F MR spectroscopy and electron microscopy showed a rapid and efficient uptake of nanoparticles by DC. The 19F signal intensity in these cells was shown to be directly related to 19F nanoparticle size. 19F/1H MRI showed that DC function was not disturbed following 19F-labeling as demonstrated by an efficient migration of these cells into draining popliteal lymph nodes.