S. David Smith1, June-Hee Park2, Michael Ma3, Mei Yu4, Shaonan Zhang3, Mirjana Maletic-Savatic5, Annemie Van der Linden6, G. Enikolopov2, Helene Benveniste4
1Medical, Brookhaven National Laboratory, Upton, NY, United States; 2Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, United States; 3Stony Brook University, United States; 4Anesthesiology, Stony Brook University, Stony Brook, NY, United States; 5Baylor College of Medicine, TX, United States; 6University of Antwerpen, Antwerpen, Belgium
We tested the hypotheses that 1) the electroconvulsive shock (ECS) induced increase in the 1.28ppm neural stem cell biomarker could be tracked using LCModel software and 2) that ECS being an effective anti-depressant treatment would result in changes in glutamate detectable by LCModel software. Analysis of our 1HMRS data acquired from the rat dentate gyrus before and after ECS demonstrated that the 1.28ppm signal was too low to be tracked by LCModel software (CRLB<30%). Further we show that glutamate increases significantly in rats exposed to ECS suggesting a role for glutamate as an anti-depressant and/or as an important instigator of synaptic plasticity.