Hirohiko Imai1,2, Atsuomi Kimura2, Takuya Kitao2, Emi Okubo2, Yuki Hori2, Satoshi Iguchi2, Hideaki Fujiwara2
1Center for Advanced Research and Education in Drug Discovery and Development, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan; 2Department of Medical Physics and Engineering, Division of Health Sciences, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
Several techniques in hyperpolarized 129Xe MRI and MRS were applied to spontaneously breathing mice for comprehensively evaluating pulmonary structures and functions in emphysematous mice. The abnormalities of pulmonary structure, perfusion and ventilation were successfully detected in emphysematous mice, while significant difference was not shown in gas exchange. Although further improvement was needed for evaluating gas exchange in spontaneous breathing condition, it was shown that the method described here could become useful for drug research and development using small rodents since this protocol was able to detect several important pathological changes non-invasively.