Malte Frederick Alf1,2,
Hongxia Lei1,3, Carole Berthet4, Lorenz Hirt4,
1Laboratory of Functional & Metabolic Imaging, Ecole Polytechnique Fdrale de Lausanne, Lausanne, Switzerland; 2Institute of Pharmaceutical Sciences, ETH Zrich, Zrich, Switzerland; 3Department of Radiology, University of Lausanne; 4Department of Clinical Neuroscience, Centre Hospitalier Universitaire Vaudois
We developed a 1H-MRSI protocol on a 14.1T magnet to investigate the localization of changes in the mouse neurochemical profile after 30 minutes of transient ischemia (MCAO model). Maps with 1.4 microliter effective resolution were acquired, including e.g. separate glutamate and glutamine and the, to our knowledge, first in vivo mapping of GABA and glutathione. Metabolite acquisition time was 45 minutes. Lactate and NAA concentration changed in core and penumbra; cholines and glutathione in the entire MCA-territory. Glutamine was elevated in ischemic striatum and cortex until 8h/24h after reperfusion respectively, indicating differences in excitotoxic effects and secondary energy failure.