Anup Singh1, Kejia Cai1, Mohammad Haris1, Joel H. Greenberg2, Hari Hariharan1, Ravinder Reddy1
1CMROI, Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA; 2Department of Neurology, University of Pennsylvania, Philadelphia, PA, USA
Dependence of CEST effect from amine protons of Glutamate (GluCEST) on pH is demonstrated using phantom and in-vivo rat data. While GluCEST has a non-linear dependence on pH from 1-8 it appear to vary linearly over a physiologically relevant pH changes between 6 and 7.4. In the ipsilateral side of middle cerebral artery occlusion (MCAO) model of stroke in rat brain, GluCEST is almost doubled at 4.5 hrs after occlusion compared to contralateral side.