1University Halle-Wittenberg, Halle, Germany; 2Johns Hopkins University School of Medicine, Baltimore, MD, USA; 3Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA; 4Russell H. Morgan Department of Radiology & Radiological Science, Johns Hopkins University Shool of Medicine, Baltimore, MD, USA; 5JHU ICMIC Program, Russell H. Morgan Department of Radiology & Radiological Science, Johns Hopkins University, Baltimore, MD, USA
Multi-scale characterization of angiogenesis in pre-clinical breast cancer models helps elucidate its role in tumor progression and facilitates investigations into the systems biology of angiogenesis. By imaging the tumor vasculature with three independent methods (in vivo MRI, ex vivo micro-CT and MR-microscopy), we acquired complementary data that yields information about tumor angiogenesis at different spatial scales. The extracted vasculature and fractional blood volume maps computed from these complementary datasets showed excellent agreement over all spatial scales. In addition to characterizing breast cancer angiogenesis, we use these multi-scale data in biophysical models of MR image contrast, and computational models of angiogenesis.