In Vivo Absolute Quantification for Mouse Muscle Metabolites using an Inductively Coupled Synthetic Signal Injection Method & Newly Developed 1H/31P Dual Tuned Probe

Donghoon Lee¹, Kenneth Marro¹, Mark Mathis¹, Eric Shankland¹, Cecil Hayes¹

We obtained robust estimates of 31P metabolite content in mouse muscles using a synthetic signal injection method and an optimized, 1H/31P dual tuned probe. 31P metabolite concentrations obtained from normal mice were well within the expected range reported in the literature. Our 1H/31P dual tuned probe was composed of two separate solenoid coils and each solenoid included a second coil to allow injection of pseudo signals. In addition to in vivo 31P metabolite quantitation, 1H MR imaging was conducted for both legs to perform parametric MRI along with muscle volume measurements.