Michael Samuel Dodd1, Beat Schuler1, Vicky Ball1, Daniel Ball1, George K. Radda1, Houman Ashrafian2, Hugh Watkins2, Kieran Clarke1, Damian J Tyler1
1Physiology, Anatomy & Genetics, Oxford University, Oxford, United Kingdom; 2Cardiovascular Medicine, Oxford University, Oxford, United Kingdom
Alterations in cardiac metabolism underlie many diseases of the heart. The advent of cardiac hyperpolarized magnetic resonance spectroscopy, via dynamic nuclear polarization, has enabled a greater understanding of the in vivo metabolic changes seen as a consequence of heart disease. This study demonstrates for the first time a slice selective approach to investigate the in vivo metabolism of [1-13C]pyruvate in the murine heart. There is a significant 64% reduction in PDH flux in the fasted murine heart, similar to previous findings in the rat. This work validates the method for detecting changes in transgenic murine models of cardiovascular disease.