Donghan Yang1, William M. Spees2,
Joseph J. H. Ackerman1,2, Philip Verghese3, David M.
Holtzman3, Jeff J. Neil2,3
1Department of Chemistry,
Washington University in St. Louis, St. Louis, MO, United States; 2Department
of Radiology, Washington University in St. Louis, St. Louis, MO, United
States; 3Department of Neurology, Washington University in St.
Louis, St. Louis, MO, United States
High-resolution (59 59 250 m) T2W images at 11.74 Tesla were acquired to study the early stage of CNS injury in mice following hypoxia-ischemia at postnatal day 7. Binomial-series water/fat-frequency-selective excitation pulses were employed to eliminate chemical-shift artifact from fat signal. Phantom tests were conducted to evaluate this fat-suppression method. In mice, T2 hyperintensity was detected specifically in the striatum and hippocampus in injured hemisphere as early as 6 hours after hypoxia. Unexpectedly, the full extent of injury was apparent within 24 hours.