Eliana Gianolio1, Valeria Menchise2, Giuseppe Digilio3, Evelina Cittadino4, Carla Carrera4, Valeria Catanzaro4, Silvio Aime4
1Universit di Torino,
Mammalian cells display a large number of reactive protein thiols on the extracellular side of the plasma membrane (Exofacial Protein Thiols, EPTs). These EPTs are quite reactive and can be chemically labeled by suitably designed MRI contrast agents. Compound Gd-DO3A-PDP can efficiently label B16 melanoma cells both ex vitro and in vivo (xenografted tumor) as a function of the levels of EPTs shown by cells.