Takashi Watanabe1, Jens Frahm1, Thomas Michaelis1
1Biomedical NMR Research
GmbH, Max Planck Institute for Biophysical Chemistry,
This work demonstrates the combined and complimentary use of MnCl2 and Gd-DTPA to improve the white/gray matter contrast in magnetization-transfer MRI (3D FLASH) of the brain of living mice. A systemic Mn2+ administration followed by an intraventricular Gd-DTPA administration increased the SNR by 26% in the white and by 46% in the gray matter, which resulted in a CNR improvement by 121%. The underlying T1 shortening was more pronounced in the gray ( 62%) than in the white matter (39%). Intracellular Mn2+ and extracellular Gd-DTPA efficiently complement each other in shortening the T1 of brain tissue fluid.