Tariq Shah1, Balaji Krishnamachary1, Flonne Wildes1, Yelena Mironchik1, Zaver M. Bhujwalla1
1Radiology, Johns Hopkins University, Baltimore, MD, United States
The hypoxia inducible factor (HIF) is a master regulator of hypoxia inducible genes several of which facilitate invasion and metastasis. Here, using our MR-compatible cell perfusion assay, we investigated the effect of HIF-1α and HIF-2α silencing on the ability of MDA-MB-231 breast cancer cells to invade and degrade the extracellular matrix (ECM). MDA-MB-231 HIF double knocked down cells showed a significant decrease in invasion and ECM degradation, as well as altered metabolism. These data demonstrate the importance of silencing both HIF-1 and 2α; to reduce ECM degradation and invasion in this triple negative aggressive and metastatic breast cancer cell line.