Yunzhou Shi1, David Finkle2, Franklin Peale3, Jed Ross1, Maj Hedehus1, Nicholas Van Bruggen1, Suzanna Clark2, Rayna Venook2, Sarajane Ross2, Richard Carano1
1Biomedical Imaging, Genentech Inc.(Roche group), South San Francisco, CA, United States; 2Translational Oncology, Genentech Inc.(Roche group), South San Francisco, CA, United States; 3Pathology, Genentech Inc.(Roche group), South San Francisco, CA, United States
A novel approach that combines 19F MRI oximetry with diffusion-based multispectral analysis was developed. The current study demonstrates that pO2 measurements can be restricted to the viable tumor and that the necrotic tissue classes contribute erroneous data to whole-tumor estimates of the pO2 response during a breathing gas challenge experiment on mice. This novel approach provides a means to measure pO2 within tissue of therapeutic interest and address the issue of tumor heterogeneity that complicates pO2 tumor imaging.