Kai-Hsiang Chuang1, Fatima A. Nasrallah1, Guilhem Pages2, Philip William Kuchel2, Xavier Golay3
1MRI Group, Singapore Bioimaging Consortium, Singapore, Singapore; 2Mechanistic Systems-biology NMR Group, Singapore Bioimaging Consortium, Singapore, Singapore; 3Institute of Neurology, University College of London, United Kingdom
1H magnetic resonance imaging (MRI) coupled with chemical exchange saturation transfer (CEST) was used to measure 2-deoxy-D-glucose (2DG) and 2DG 6-phosphate (2DG6P) in the brains of anaesthetized rats. The gluco-CEST signal was compared with 2DG6P measured in vivo with 31P MRS under different levels of isoflurane and hypercapnia. Gluco-CEST reflected cerebral metabolism but the data needed to be interpreted with caution to account for signals variations that occurred with the initial concentration of glucose, pH and due to the time-dependent washout of glucose.