Yutong Liu1, 2, Adrian A. Epstein2, Aditya N. Bade2, Howard E. Gendelman2, Michael D. Boska1, 2
1Department of Radiology, University of Nebraska Medical Center, Omaha, NE, United States; 2Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, United States
We investigated manganese (Mn) glial uptake In Vitro and in vivo (rodents) to assess the roles of glia and neurons in MEMRI signal enhancement. PC 12 cells were co-cultured with activated astrocytes and microglia in the In Vitro study. In-vivo, glial activation was induced by LPS injection. In Vitro Mn uptake by PC 12 cells was dependent on the concentrations of Mn and glial activation. The amount of Mn in glia was not affected by activation or Mn concentration. in vivo, no significant signal enhancement was detected using T1 mapping by glial activation.