Daniel Ball1, Rachel Cruickshank1, Carolyn Carr1, Daniel Stuckey2, Kieran Clarke1, Damian Tyler1
1Physiology, Anatomy and Genetics, Oxford University, Oxford, Oxfordshire, United Kingdom; 2Imperial College, London, United Kingdom
The aim of this work was to demonstrate the use of a combined dynamic nuclear polarization and chemical shift imaging protocol in the isolated perfused heart to image metabolism in the setting of both acute and chronic myocardial ischemia. The imaging protocol was successfully applied in both models, and in the acute ischemia model, metabolic disturbances could be clearly visualised. Future work will focus on the translation of these techniques into in vivo models of ischemia.