Jannie P. Wijnen1, 2, Lu Jiang1, Wybe J.M. van der Kemp2, Dennis W.J. Klomp2, Kristine Glunde1
1Johns Hopkins University in vivo Cellular and Molecular Imaging Center,Russell H. Morgan Department, Johns Hopkins University School of Medicine, Baltimore, MD, United States; 2Department of Radiology, University Medical Centre Utrecht, Utrecht, Netherlands
Here we have demonstrated that using polarization transfer (PT) methods such as refocused insensitive nuclei enhanced by polarization transfer (RINEPT) and its adiabatic version (BINEPT) at 7 and 9.4Tesla can improve the 31P magnetic resonance spectroscopy (MRS) detection of phosphomono- and diesters in human breasts and breast tumor xenograft models in vivo. BINEPT 31P MRS was able to detect partially resolved phosphoethanolamine, phosphocholine, glycerophosphoethanolamine and glyerophosphocholine because it is insensitive to unwanted broad resonances from macromolecules that do not posses H-P J-coupling, without compromising SNR compared to direct 31P MRS.