Xing Chen1, Peter Boesiger1, Anke Henning1
1Institute for Biomedical Engineering, University and ETH Zurich, Zurich, Switzerland
Localized natural abundance 13C MRS provides an investigative tool for studying metabolism. Polarization transfer methods like DEPT can be used to enhance sensitivity. The combination of DEPT with proton localized PRESS avoids the large chemical shift displacement and enables tissue specific investigation of lipid composition. However, the concurrent strong homonuclear proton scalar coupling in many metabolites modifies the proton spin coherence distribution and therefore leads to a substantial reduction in the 13C signal enhancement. In this work, J-refocused PRESS localized DEPT is introduced to suppress this effect and enable simultaneous and tissue specific DEPT enhancement of multiple fatty acid resonances.