Quantification of Fatty Acids in Human Calf Adipose Tissue and Muscle by 13C MRS Using J-Refocused PRESS DEPT and ERETIC

Xing Chen¹, Peter Boesiger¹, Anke Henning², ³

A J-refocused proton PRESS localized DEPT sequence is applied to achieve reliably localized and SNR enhanced signals by in vivo 13C MRS using volume coil, with the combination of optically transmitted and inductively coupled ERETIC as a reference for absolute quantification. The proposed method is used to assess the fatty acids from human deeper locations: calf tibial bone marrow and skeletal muscle, among omnivores, vegetarians and vegans. Both relative percentages and absolute molar concentrations are evaluated and analyzed. The results demonstrate the applicability of quantitative, SNR enhanced and localized 13C MRS for large scale noninvasive investigation of the impact of dietary intake, physical exercise or pathology on fatty acid metabolism and related diagnosis.