Yen-Yu Ian Shih1, 2, Bryan H. De La Garza2, Shiliang Huang2, Guang Li2, Lin Wang3, Timothy O. Duong4
1Experimental Neuroimaging Laboratory, Department of Neurology and Biomedical Research Imaging Center, University of North Carolina, Chapel Hill, NC, United States; 2Research Imaging Center, University of Texas Health Science Center at San Antonio, San Antonio, TX, United States; 3Devers Eye Institute, Legacy Research Institute, Portland, OR, United States; 4Research Imaging Center, UT Health Science Center at San Antonio, San Antonio, TX, United States
Continuous arterial spin labeling (CASL) MRI, widely used to non-invasively image blood flow (BF) of the brain, has been applied to image BF of the rat brain and retina but remains to be validated. The goal of this study was to use an established microsphere technique to cross-validate ASL MRI BF measurement in the rat brain and retina. By using a mixture of the two different sized fluorescent microspheres with two different colors, the cerebral, retinal and choroidal BF can be measured simultaneously in the same subject. Our data showed that BF values by MRI are in good accordance with the microsphere technique in the rat retina and cerebral cortex.