Michael Zhao1, Esther Myint2, Geoffrey Watson3, Roger Bourne1
1University of Sydney, Sydney, NSW, Australia; 2Laverty Pathology, Sydney, NSW, Australia; 3Royal Prince Alfred Hospital, Sydney, NSW, Australia
Diffusion weighted microimaging of fixed prostate tissue reveals distinct diffusivity differences between epithelium, stroma, and acinal spaces1. In normal glandular tissue changes in the relative volumes of epithelium and stroma explain ~60% of the variation in signal fraction of a biexponential model of diffusion signal decay. We hypothesise that microscopic tissue volume changes may also explain the observed strong correlation between cancer Gleason grade and apparent diffusion coefficient measured in vivo. In this pilot study we used semi-automated morphometry methods to quantify partial volumes of epithelium, stroma and acinal space in H&E-stained histological images of benign and cancerous prostate tissue and compared these results with segmentation and statistical analysis of diffusion weighted microimages of normal tissue.